Isoform specificity of progesterone receptor antibodies
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چکیده
منابع مشابه
Isoform specificity of progesterone receptor antibodies
Progesterone receptors (PR) are prognostic and predictive biomarkers in hormone-dependent cancers. Two main PR isoforms have been described, PRB and PRA, that differ only in that PRB has 164 extra N-terminal amino acids. It has been reported that several antibodies empirically exclusively recognize PRA in formalin-fixed paraffin-embedded (FFPE) tissues. To confirm these findings, we used human ...
متن کاملModulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium
Background. Regulation of myometrial progesterone receptor (PR) expression is an unresolved issue central to understanding the mechanism of functional progesterone withdrawal and initiation of labor in women. Objectives. To determine whether pregnant human myometrium undergoes culture-induced changes in PR isoform expression ex situ and, further, to determine if conditions approaching the in vi...
متن کاملProgesterone receptor isoform identification and subcellular localization in endometrial cancer.
OBJECTIVE These studies were undertaken to characterize the subcellular localization of the two major isoforms of progesterone receptors (PR), PRA and PRB, in endometrial cancer. METHODS Immunohistochemistry, immunoprecipitation, and confocal microscopy were performed using Hec50co and KLE endometrial cancer cell models expressing PRA or PRB as a consequence of transduction. The location of P...
متن کاملMonoclonal antibodies to rabbit progesterone receptor: crossreaction with other mammalian progesterone receptors.
A mouse was immunized with purified rabbit uterine cytosolic progesterone receptor (specific activity: 3 nmol of steroid bound per mg of protein). After fusion of its spleen cells with Sp2-OAg myeloma cells, supernatants of 11 hybrid cultures were found to react in both an immunoenzymatic test and a double-immunoprecipitation test with the progesterone receptor. Clones were obtained from the fi...
متن کاملNovel monoclonal antibodies against human uterine progesterone receptor. Mapping of receptor immunogenic domains.
Progesterone receptor was purified in a single step from human uteri using immunoaffinity chromatography with monoclonal antibodies raised against the rabbit receptor. A total of 39 monoclonal antibodies were prepared against the human receptor and characterized. Immunoblot experiments using crude uterine cytosol or purified receptor showed that the antibodies belonged to three groups: they rec...
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ژورنال
عنوان ژورنال: The Journal of Pathology: Clinical Research
سال: 2017
ISSN: 2056-4538
DOI: 10.1002/cjp2.83